Mitomycin C-Activity Effected by Vitamins B1, C, E and -Carotene under Irradiation with γ-Rays

Edith Heinrich and Nikola Getoff* Institute for Theoretical Chemistry and Structural Biology as well as Ludwig Boltzmann Institute for Radiation Chemistry and Radiation Biology, The University of Vienna, Althanstr.14, A-1090 Vienna, Austria. Fax: ++43(1)-4277-52795. E-mail: [email protected] * Author for correspondence and reprint requests Z. Naturforsch. 58c, 244Ð248 (2003); received October 11/November 7, 2002 Vitamin B1 (thiamine) can essentially effect the activity of mitomycin C (MMC), added individually or in combination with antioxidant vitamins (C, E-acetate, -carotene) as found in experiments in vitro (Escherichia coli bacteria, AB 1157) under irradiation with γ-rays. The environment plays a crucial role. In airfree media vitamin B1 leads to a 2-fold increase of the MMC-efficiency, but adding vitamin C it decreases. In the presence of all vitamins (B1, C, E-ac., and -carotene) the MMC-action increases about 1.8-fold. In aerated media vitamin B1 causes an about 4-times increase of the MMC-efficiency, but by adding vitamin B1 and C the MMC-activity decreases by a factor of two, whereas in the presence of B1, C, E-ac., and -carotene it rises again to 2.6-fold. In environment saturated with N2O (conversion of eaq into OH radicals) a different picture is observed. The presence of vitamin B1 or vitamin B1 + C causes a strong decrease of the MMC-efficiency, but the addition of all vitamins (B1, C, E-ac., and -car.) leads to a small increase of the cytostatic action. The results demonstrate the influence of vitamin B1 used individually or in combination with other antioxidants on the MMC-efficiency and the strong effect of the environment. The results are of interest for the application of MMC in radiotherapy.